cgtase. Organism. Anaerobranca gottschalkii. Status. Unreviewed-Annotation score: -Protein inferred from homology i. Function i Cofactor i. Ca 2+ ARBA annotation. GO
Cyclodextrin glycosyltransferase (CGTase) is an enzyme of the alpha-amylase family, which uses a double displacement mechanism to process alpha-linked glucose polymers. We have determined two X-ray structures of CGTase complexes, one with an intact substrate at 2.1 A resolution, and the other with a covalently bound reaction intermediate at 1.8 A resolution.
Organism. Anaerobranca gottschalkii. Status. Unreviewed-Annotation score: -Protein inferred from homology i.
In addition, we have revealed the key do-mains responsible for its hydrolytic activity and resistance induction. Further experiments demonstrated that CGTase is a potential func - CGTase is an important industrial enzyme that can transfer starch to glycosyl groups to form cyclodextrin (Li et al., 2014c; Qi et al., 2007). CGTase has been found in many bacteria, including Bacillus spp. (Chen et al., 2018; Gimenez et al., 2019; Yap et al., 2010) and Paenibacillus spp. (Castillo et al., 2018).
2016年1月13日 （注：CGTaseはCDを作る酵素ですが、壊す反応も触媒してしまいます！） そこで、包接複合体形成時のd-CDの分子構造を計算化学的手法により作成し比較 検討しました。その結果
Ca 2+ ARBA annotation. GO 糖質科学のことば / Saccharide-B05J. α-アミラーゼファミリーの概念. 澱粉は基本的にα-1,4結合のみからなるグルコースポリマーであるアミロースと、α-1,4結合でつながったグルコースポリマーがα-1,6結合で分岐した構造のアミロペクチンとから構成される。.
In the study, we investigated the contribution of Ca 2+ to the thermostability of α-cyclodextrin glycosyltransferase (α-CGTase) from Paenibacillus macerans, which has two calcium-binding sites (CaI and CaII), and β-CGTase from Bacillus circulans, which contains an additional calcium-binding site (CaIII), consisting of Ala315 and Asp577.
GO - Molecular function i. alpha-amylase activity Source: InterPro γ-CGTase activity increased, in both control cultures (from 71.7 to 97.0%) and cultures supplemented with β-cyclodextrin (from 56.3 to 91.8%). More importantly, the effect of β-cyclodextrin on total soluble γ-CGTase activity, as a multiple of the control value, increased CGTase enzyme production is depicted in Figs 2 and 3 for Bacillus sp. and B. circulance, respectively. The CGTase activity in culture broths was observed increasing with increase in initial inoculum level for Fig. 1⎯CGTase production pattern in Bacillus sp. and B. circulance during growth phase Cyclodextrin glycosyltranferase (CGTase EC 2.4.1.19) is a bacterial enzyme that converts starch and other 1,4-linked α-glucans to cyclodextrins (closed-ring structures composed mainly of 6, 7 and 8 glucosyl units, named α, β and γ-cyclodextrin, respectively) (French, 1957; Thoma and Stewart, 1965).
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CGTase, was selected from B. cereus YUPP-10 by a constructed fos-mid library. We discovered that CGTase has antimicrobial activity and induces resistance. In addition, we have revealed the key do-mains responsible for its hydrolytic activity and resistance induction. Further experiments demonstrated that CGTase is a potential func - Cyclodextrin glycosyltransferase (CGTase, EC 2.4.1.19) is a carbohydrate‐active enzyme that belongs to glycosyl hydrolase family 13. CGTase can catalyse hydrolysis reactions, cleaving the linkages in the starch molecule (van der Veen et al., 2000).
β-cyclodextrin glucosyl transferase (β-CGTase) hydrolyses starch to produce β-cyclodextrin by transglycosylation (cyclization) activity. The conventional method for detection of β-CGTase
Cyclodextrin glucanotransferase (CGTase; EC 2.4.1.19) is a unique member of glycoside hydrolase family 13. Its defining property is the ability to form cyclodextrins from starch, through an intramolecular transglycosylation reaction (cyclization).
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Ca2+ affected β-CGTase thermostability significantly. After Ca2+ was added to β-CGTase solution to a final concentration of 5 mM followed by incubation for 120 min at 60 °C, residual activity of β-CGTase was 88.3%, which was much higher than that without Ca2+. However, Ca2+ had a small contribution to α-CGTase thermostability.
cyclomaltodextrins (Schardinger dextrins) of various sizes (6,7,8, etc. glucose units) are formed reversibly from starch and similar substrates.
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シクロデキストリン (英: cyclodextrin) は数分子の D - グルコース が、 α-1,4グリコシド結合 によって結合し環状構造をとった環状 オリゴ糖 の一種である 。
In addition, we have revealed the key do-mains responsible for its hydrolytic activity and resistance induction. Further experiments demonstrated that CGTase is a potential func - CGTase is an important industrial enzyme that can transfer starch to glycosyl groups to form cyclodextrin (Li et al., 2014c; Qi et al., 2007). CGTase has been found in many bacteria, including Bacillus spp. (Chen et al., 2018; Gimenez et al., 2019; Yap et al., 2010) and Paenibacillus spp. (Castillo et al., 2018).